🌱Emerging

PE-22-28 (Mini-Spadin)

Also known as: Mini-Spadin

CognitiveAnti-Aging
Not FDA Approved

Primary recreational use: Cognitive and mood enhancement

What is PE-22-28 (Mini-Spadin)?

PE-22-28, or Mini-Spadin, is a heptapeptide derived from the parent peptide Spadin, developed for its potent antagonism of the TREK-1 channel. Known for rapid antidepressant effects, it enhances neurogenesis and synaptogenesis, potentially benefiting mood disorders. The peptide remains unapproved by the FDA and is primarily available as a research compound.

Primary Benefits

Rapid antidepressant effects. Enhances neurogenesis. Improves synaptogenesis.

How It Works

PE-22-28 acts by selectively blocking the TREK-1 potassium channels, which increase neuronal excitability and enhance serotonin neurotransmission. This inhibition leads to the activation of CREB and BDNF, promoting neurogenesis in the hippocampus.

Safety Tips

Precautions reported in community discussions and literature. This is not a complete list.

  • 1.DON'T exceed research protocol dosing ranges.
  • 2.DON'T combine with MAOIs or high-dose SSRIs due to serotonin syndrome risk.
  • 3.DON'T use if you are pregnant or breastfeeding.
  • 4.AVOID using poor quality peptides lacking third-party testing.
  • 5.NEVER shake the solution violently during reconstitution.

Who Should NOT Use

  • Pregnancy
  • Breastfeeding

Use Contexts

Different purposes have different protocols, dosing, and considerations

🔬Investigational

Depression research

moderate evidence

PE-22-28 is studied for its rapid antidepressant effects and potential neurogenesis enhancement.

Evidence: Preclinical studies show significant antidepressant effects with enhanced neurogenesis.

The following are ranges reported in published literature and community discussions. These are NOT recommendations or medical advice.

Reported Range
50-200 mcg
Reported Frequency
once daily
Reported Cycle
4-8 weeks
Reported Route
Subcutaneous

Timing: Morning dosing

Notes: Comparable to preclinical dosing adjusted for human equivalency.

Community-Reported Protocols

Not recommendations: The following protocols are compiled from online communities and published literature. Pepbase does not endorse, recommend, or verify these protocols. They are provided for informational purposes only. Individual responses vary significantly. Consult a licensed medical professional before any use.

Reported GoalReported DoseReported FrequencyReported Route
Preclinical Antidepressant Study3-4 mcg/kgOnce dailyIntraperitoneal (animal model)

Pharmacokinetics

Half-Life
~23 hours
Time to Peak
4 days
Clearance
Clearance information based on animal data.

Reported Side Effects

This is not a complete list. Other side effects may occur.

Common
None specific reportedreported (frequency unknown)
Serious / Rare
  • Potential serotonin syndrome.
Injection-Specific Warnings
  • Use sterile technique to avoid infections.

Storage

Temperature
Refrigerate at 2-8°C
Reconstituted
Refrigerate, use within 4-6 weeks
Shelf Life
Typically 4-6 weeks post-reconstitution
Light Sensitive
Yes - protect from light

Harm Reduction Information

Quality Concerns

  • Quality may vary by supplier; ensure third-party testing.
  • Poor storage conditions can degrade peptide.

Safety Tips

  • Start with the lowest effective dose.
  • Use sterilized water for reconstitution.
  • Avoid combining with contraindicated drugs like MAOIs.
  • Use sterile technique for injections.
  • Rotate injection sites to prevent tissue damage.

Warning Signs to Stop

  • Unexpected allergic reactions.
  • Signs of serotonin syndrome like confusion or agitation.

Seek Medical Help Immediately If

  • If serotonin syndrome symptoms occur.

Frequently Asked Questions

Common questions about PE-22-28 (Mini-Spadin)

How long until I see effects?

PE-22-28 shows measurable antidepressant effects within 4 days according to preclinical models.

What time of day should I take it?

PE-22-28 is usually administered in the morning, with a duration of action around 23 hours.

How can I minimize side effects?

To minimize side effects, use PE-22-28 at the lowest effective dose and avoid combining it with contraindicated drugs like MAOIs.

Does tolerance develop?

Information on tolerance development with PE-22-28 is limited, so users should adhere to cycling protocols.

How should I store it?

Store PE-22-28 in the refrigerator at 2-8°C, and use reconstituted solutions within 4-6 weeks.

Reported Combinations

Compounds reported to be used alongside PE-22-28 (Mini-Spadin) in community discussions

investigational

Semax

Potential synergistic effects on cognitive and mood enhancements.

investigational

Cerebrolysin

May enhance neurotrophic benefits.

investigational

BPC-157

Compatible and potentially synergistic for neuroprotection.

Reported Interactions

Interactions reported in literature and community discussions.

SSRIsmoderate

Increased risk of serotonin syndrome when combined.

MAOIsmajor

Potentially dangerous interaction increasing serotonin syndrome risk.

Creatineminor

No known interaction; generally considered safe.

Reconstitution Calculator

mL
U100 insulin syringe01020304050607080901001mL

100 units = 1 mL

Safety Tips

  • Always consult a licensed medical professional for dosing and reconstitution instructions.
  • Always double check your units. Mixing up mg and mcg can be very dangerous.
  • Make sure to select your syringe type. U-100 and U-50 syringes have different units.
  • Ensure you're not adding more BAC than your vial can hold. Most vials have maximum capacities listed.

This calculator is for informational and educational purposes only. It does not provide medical advice, dosing recommendations, or safety determinations. Reference ranges shown are compiled from publicly available sources and are not a substitute for guidance from a licensed healthcare professional.

Explore More Research

Browse our database of peptide research information compiled from published literature and community sources.

Browse All PeptidesAbout Pepbase App

Citations

Last updated: October 15, 2023

Important Disclaimer

AI-Generated Content Notice

The information on this page has been compiled using artificial intelligence (AI) and large language model (LLM) technology from multiple sources including published literature, online communities, and public databases. This content may contain errors, inaccuracies, outdated information, or omissions. We make no guarantees regarding the accuracy, completeness, or reliability of any information presented. You are solely responsible for independently verifying any information before relying on it.

Not Medical Advice

Nothing on this website constitutes medical advice, diagnosis, or treatment recommendations. The information provided is for educational and harm-reduction purposes only. We do not provide individualized dosing guidance or treatment recommendations. Protocols and dosing information shown represent reports from community discussions and published literature—they are not endorsements or recommendations. Always consult with a licensed healthcare professional before making any health-related decisions.

No Endorsement of Use

Pepbase does not endorse, encourage, or recommend the use of any peptide or substance discussed on this website. Many compounds discussed are not approved for human use by the FDA or other regulatory agencies. Some substances may be illegal to possess, sell, or use in your jurisdiction. We do not facilitate or encourage the procurement, distribution, or use of controlled or prescription-only substances. Users are solely responsible for understanding and complying with all applicable laws in their jurisdiction.

Calculators and Tools

Any calculators, estimators, or tools provided on this website are for informational purposes only and produce estimates that may be inaccurate. Errors in calculations can result in serious harm. You are solely responsible for verifying all calculations independently. These tools do not account for individual factors that may affect dosing and should never be relied upon as a substitute for professional medical guidance.

Assumption of Risk

By using this website, you acknowledge that you understand the risks associated with using unregulated substances and that you assume full responsibility for your own health decisions. Pepbase, its creators, contributors, and affiliates disclaim all liability for any harm, injury, or damages arising from the use or misuse of information on this website. Use of any information on this site is entirely at your own risk.

If you are experiencing a medical emergency, call emergency services immediately. If you have questions about medications or supplements, consult a licensed pharmacist or physician.